DNA

Part:BBa_K1667005:Design

Designed by: Mingkai Xu   Group: iGEM15_Shiyan_SY_China   (2015-09-07)

Thermo-regulated opdA Coupled with RecA-reduced ccdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 281
    Illegal AgeI site found at 476
    Illegal AgeI site found at 815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1686


Design Notes

There is a DNA spacer fragment between the two domains in this parts.


Source

The sequence of the constitutive promoter in this part is from BB_J23106 in igem. The sequence of the RNA thermometer in this part is from BB_K115017 in igem. The sequence of the opdA gene in this part is from BB_K215090, BB_K215091, and BB_K1010008 in igem. The sequence of the RecA（SOS）promoter in this part is from BB_J22106 in igem. The sequence of the ccdB gene in this part is from BB_K145151 and BB_K1010007 in igem. The sequence of the ompA in this part is from AJ617284.1 in GenBank.

References